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pparg 81b8 rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc pparg 81b8 rabbit mab
    Figure 3. IL-13 potentiates <t>PPARg-mediated</t> beige adipogenesis. (A) Immunoblot analysis of PPARg protein in Il13ra1KO control and Il13ra1RE preadipocytes treated with IL-13 for 24 hours compared to untreated controls. n=3, experiment repeated twice. (B) RT-qPCR analyses of Pparg1 and PPARg target genes in Il13ra1KO control and Il13ra1RE preadipocytes treated with/without IL- 13 for 24 hours. n=3, experiment performed 3 times, 2-way ANOVA with Tukey’s multiple comparisons test. (C) RT-qPCR analyses of Pparg and PPARg target genes in WT preadipocytes treated with vehicle, IL-13, rosi, or IL-13+rosi for 24 hours. n=3, experiment performed 4 times, one-way ANOVA with Tukey’s multiple comparisons test. (D) RT-qPCR analyses in WT cells treated with vehicle, IL-13, rosi, or IL-13+rosi for 24 hours, followed by 2-day differentiation with
    Pparg 81b8 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 672 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 672 article reviews
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    1) Product Images from "Preadipocyte IL-13/IL-13Rα1 signaling regulates beige adipogenesis through modulation of PPARγ activity"

    Article Title: Preadipocyte IL-13/IL-13Rα1 signaling regulates beige adipogenesis through modulation of PPARγ activity

    Journal: Journal of Clinical Investigation

    doi: 10.1172/jci169152

    Figure 3. IL-13 potentiates PPARg-mediated beige adipogenesis. (A) Immunoblot analysis of PPARg protein in Il13ra1KO control and Il13ra1RE preadipocytes treated with IL-13 for 24 hours compared to untreated controls. n=3, experiment repeated twice. (B) RT-qPCR analyses of Pparg1 and PPARg target genes in Il13ra1KO control and Il13ra1RE preadipocytes treated with/without IL- 13 for 24 hours. n=3, experiment performed 3 times, 2-way ANOVA with Tukey’s multiple comparisons test. (C) RT-qPCR analyses of Pparg and PPARg target genes in WT preadipocytes treated with vehicle, IL-13, rosi, or IL-13+rosi for 24 hours. n=3, experiment performed 4 times, one-way ANOVA with Tukey’s multiple comparisons test. (D) RT-qPCR analyses in WT cells treated with vehicle, IL-13, rosi, or IL-13+rosi for 24 hours, followed by 2-day differentiation with
    Figure Legend Snippet: Figure 3. IL-13 potentiates PPARg-mediated beige adipogenesis. (A) Immunoblot analysis of PPARg protein in Il13ra1KO control and Il13ra1RE preadipocytes treated with IL-13 for 24 hours compared to untreated controls. n=3, experiment repeated twice. (B) RT-qPCR analyses of Pparg1 and PPARg target genes in Il13ra1KO control and Il13ra1RE preadipocytes treated with/without IL- 13 for 24 hours. n=3, experiment performed 3 times, 2-way ANOVA with Tukey’s multiple comparisons test. (C) RT-qPCR analyses of Pparg and PPARg target genes in WT preadipocytes treated with vehicle, IL-13, rosi, or IL-13+rosi for 24 hours. n=3, experiment performed 4 times, one-way ANOVA with Tukey’s multiple comparisons test. (D) RT-qPCR analyses in WT cells treated with vehicle, IL-13, rosi, or IL-13+rosi for 24 hours, followed by 2-day differentiation with

    Techniques Used: Western Blot, Control, Quantitative RT-PCR

    Figure 4. IL-13-IL-13Ra1 increases the expression and activity of PPARg through STAT6 and p38 MAPK. (A) Left: Schematic of the one-hybrid system to assess PGC-1α coactivation on PPARg-LBD activity. AD293 cells were transfected with Gal4-PPARg-LBD and Ppargc1 expression vector, together with Gal4 binding site containing luciferase reporter and b-galactosidase
    Figure Legend Snippet: Figure 4. IL-13-IL-13Ra1 increases the expression and activity of PPARg through STAT6 and p38 MAPK. (A) Left: Schematic of the one-hybrid system to assess PGC-1α coactivation on PPARg-LBD activity. AD293 cells were transfected with Gal4-PPARg-LBD and Ppargc1 expression vector, together with Gal4 binding site containing luciferase reporter and b-galactosidase

    Techniques Used: Expressing, Activity Assay, Transfection, Plasmid Preparation, Binding Assay, Luciferase



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    Figure 3. IL-13 potentiates <t>PPARg-mediated</t> beige adipogenesis. (A) Immunoblot analysis of PPARg protein in Il13ra1KO control and Il13ra1RE preadipocytes treated with IL-13 for 24 hours compared to untreated controls. n=3, experiment repeated twice. (B) RT-qPCR analyses of Pparg1 and PPARg target genes in Il13ra1KO control and Il13ra1RE preadipocytes treated with/without IL- 13 for 24 hours. n=3, experiment performed 3 times, 2-way ANOVA with Tukey’s multiple comparisons test. (C) RT-qPCR analyses of Pparg and PPARg target genes in WT preadipocytes treated with vehicle, IL-13, rosi, or IL-13+rosi for 24 hours. n=3, experiment performed 4 times, one-way ANOVA with Tukey’s multiple comparisons test. (D) RT-qPCR analyses in WT cells treated with vehicle, IL-13, rosi, or IL-13+rosi for 24 hours, followed by 2-day differentiation with
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    retrovirus carrying pbabe puro rxra s427f antibody anti pparg 81b8  (Cell Signaling Technology Inc)
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    Figure 1. RXRA hot-spot mutations induce the PPAR signaling pathway by activating enhancer/promoters with a canonical PPAR response element. (A) JMSU-1 and 575A cells were transduced with pBABE <t>retrovirus</t> to express indicated RXRA alleles and expression confirmed by western blot (top) or RT- qPCR in triplicate ± SD (data expressed as a fraction of actin signal). (B) Protein coding transcripts up-regulated greater than or equal to twofold Figure 1 continued on next page
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    Figure 3. IL-13 potentiates PPARg-mediated beige adipogenesis. (A) Immunoblot analysis of PPARg protein in Il13ra1KO control and Il13ra1RE preadipocytes treated with IL-13 for 24 hours compared to untreated controls. n=3, experiment repeated twice. (B) RT-qPCR analyses of Pparg1 and PPARg target genes in Il13ra1KO control and Il13ra1RE preadipocytes treated with/without IL- 13 for 24 hours. n=3, experiment performed 3 times, 2-way ANOVA with Tukey’s multiple comparisons test. (C) RT-qPCR analyses of Pparg and PPARg target genes in WT preadipocytes treated with vehicle, IL-13, rosi, or IL-13+rosi for 24 hours. n=3, experiment performed 4 times, one-way ANOVA with Tukey’s multiple comparisons test. (D) RT-qPCR analyses in WT cells treated with vehicle, IL-13, rosi, or IL-13+rosi for 24 hours, followed by 2-day differentiation with

    Journal: Journal of Clinical Investigation

    Article Title: Preadipocyte IL-13/IL-13Rα1 signaling regulates beige adipogenesis through modulation of PPARγ activity

    doi: 10.1172/jci169152

    Figure Lengend Snippet: Figure 3. IL-13 potentiates PPARg-mediated beige adipogenesis. (A) Immunoblot analysis of PPARg protein in Il13ra1KO control and Il13ra1RE preadipocytes treated with IL-13 for 24 hours compared to untreated controls. n=3, experiment repeated twice. (B) RT-qPCR analyses of Pparg1 and PPARg target genes in Il13ra1KO control and Il13ra1RE preadipocytes treated with/without IL- 13 for 24 hours. n=3, experiment performed 3 times, 2-way ANOVA with Tukey’s multiple comparisons test. (C) RT-qPCR analyses of Pparg and PPARg target genes in WT preadipocytes treated with vehicle, IL-13, rosi, or IL-13+rosi for 24 hours. n=3, experiment performed 4 times, one-way ANOVA with Tukey’s multiple comparisons test. (D) RT-qPCR analyses in WT cells treated with vehicle, IL-13, rosi, or IL-13+rosi for 24 hours, followed by 2-day differentiation with

    Article Snippet: Five μg of chromatin was used for each ChIP reaction that included either the negative control normal rabbit IgG (Cell Signaling #2729), PPARg (81B8) rabbit mAb (Cell Signaling #2443), or acetyl-histone H3 (Lys27) (D5E4) XP rabbit mAb (Cell Signaling #8173).

    Techniques: Western Blot, Control, Quantitative RT-PCR

    Figure 4. IL-13-IL-13Ra1 increases the expression and activity of PPARg through STAT6 and p38 MAPK. (A) Left: Schematic of the one-hybrid system to assess PGC-1α coactivation on PPARg-LBD activity. AD293 cells were transfected with Gal4-PPARg-LBD and Ppargc1 expression vector, together with Gal4 binding site containing luciferase reporter and b-galactosidase

    Journal: Journal of Clinical Investigation

    Article Title: Preadipocyte IL-13/IL-13Rα1 signaling regulates beige adipogenesis through modulation of PPARγ activity

    doi: 10.1172/jci169152

    Figure Lengend Snippet: Figure 4. IL-13-IL-13Ra1 increases the expression and activity of PPARg through STAT6 and p38 MAPK. (A) Left: Schematic of the one-hybrid system to assess PGC-1α coactivation on PPARg-LBD activity. AD293 cells were transfected with Gal4-PPARg-LBD and Ppargc1 expression vector, together with Gal4 binding site containing luciferase reporter and b-galactosidase

    Article Snippet: Five μg of chromatin was used for each ChIP reaction that included either the negative control normal rabbit IgG (Cell Signaling #2729), PPARg (81B8) rabbit mAb (Cell Signaling #2443), or acetyl-histone H3 (Lys27) (D5E4) XP rabbit mAb (Cell Signaling #8173).

    Techniques: Expressing, Activity Assay, Transfection, Plasmid Preparation, Binding Assay, Luciferase

    KEY RESOURCES TABLE

    Journal: Cell stem cell

    Article Title: PRDM16 maintains homeostasis of the intestinal epithelium by controlling region-specific metabolism

    doi: 10.1016/j.stem.2019.08.017

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Enteroid formation was scored on d7. table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rb anti Cleaved Notch1 (Val1744) (D3B8) Cell Signaling RRID:AB_2153348 Rb anti Cleaved Caspase 3 (Asp175) (5A1E) Cell Signaling RRID: AB_2070042 Ms anti E-Cadherin BD Transduction RRID:AB_397580 Rb anti KI67 (SP6) Abcam RRID:AB_302459 Gt anti Lysozyme C Antibody (W-20) Santa Cruz RRID:AB_2138793 (Disc) Rb anti Muc2 (H-300) Santa Cruz RRID:AB_2146667 (Disc) Ms anti-p53 (1C12) Cell Signaling RRID:AB_331743 Gt anti GFP Abcam RRID:AB_305643 Rb anti Prdm16 P. Seale Made in house Rb anti Prdm16 R and D RRID:AB_10717965 Ms anti GAPDH (GA1R) Thermo RRID:AB_10977387 Ms anti Tubulin (DM1A) Sigma RRID:AB_477583 Rb anti Pparg (H-100) Santa Cruz RRID:AB_654710 Rb anti Pparg (81B8) Cell Signaling RRID:AB_823598 Rb anti Ppara (H-98) Santa Cruz RRID:AB_2165737 Rb anti Lysozyme C Agilent RRID:AB_2341230 Rb anti Chromogranin A Abcam RRID:AB_301704 Rb anti MUC2 Cloud Clone RRID:AB_2811028 Rb anti RFP Rockland RRID:AB_2209751 Ms anti IFNAR1 (MAR15A3) Leinco Tech.

    Techniques: Transduction, Recombinant, Plasmid Preparation, Sample Prep, SYBR Green Assay, Multiplex Assay, Purification, Lysis, Isolation, Autoradiography, RNA Sequencing Assay, ChIP-sequencing, Real-time Polymerase Chain Reaction, Sequencing, Software, Expressing

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    Journal: Cell metabolism

    Article Title: A PRDM16-driven metabolic signal from adipocytes regulates precursor cell fate

    doi: 10.1016/j.cmet.2019.05.005

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rb anti PRDM16 P. Seale Generated in house Rb anti PPARg (81B8) Cell Signaling 2443S Ms anti Tubulin (DM1A) Sigma T6199 Ms anti Actin, clone C4 Millipore MAB1501 Ms anti Actin, α-Smooth Muscle Sigma A2547 Ms anti TGFβ 1, 2, 3 Novus Biologicals MAB1835-SP Ms anti SMAD3 Santa Cruz Sc-101154 Rb anti P-SMAD3 AbCam ab52903 Rb anti HIF1α Cayman Chemical 10006421 Rb anti Perilipin Cell Signaling 3470S Rb anti RFP VWR Scientific RL600-401-379 APC anti-mouse PDGFRα BioLegend 136007 Goat anti-P-SMAD2/3 Santa-Cruz Sc-11769 Chemicals, Peptides, and Recombinant Proteins Tamoxifen (Free Base) Sigma T5648 4-hydroxy-tamoxifen Sigma H6278 Etomoxir Sigma E1905 Corn Oil Sigma C8267 Palmitic Acid Sigma 76119 Methanol (HPLC) VWR MX0475-1 Water (HPLC) Fisher Chemical W5-1 Palmitic Acid [9,10-3H(N)], 1mCi Perkin Elmer NET043001MC DMOG Cayman Chemical 71210 Recombinant Mouse TGFβ R & D 7345-B2 ion-exchange columns Bio-Rad 7316211 Palmitic Acid (1-13C, 99%) Cambridge Isotopes CLM-150 Sodium-3-hydroxybutyrate Sigma 54965 TruSeq RNA Sample Prep Kit v2 set A Illumina RS-122-2001 PCR Master Mix, Power SYBR Green Applied Biosystems 4367659 CL 316, 243 Sigma C5976 Deposited Data RNA sequencing data -Young vs.

    Techniques: Generated, Recombinant, Sample Prep, SYBR Green Assay, RNA Sequencing Assay, Mouse Assay, Software

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    Journal: Cell metabolism

    Article Title: A PRDM16-driven metabolic signal from adipocytes regulates precursor cell fate

    doi: 10.1016/j.cmet.2019.05.005

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Rb anti PPARg (81B8) , Cell Signaling , 2443S.

    Techniques: Generated, Recombinant, Sample Prep, SYBR Green Assay, RNA Sequencing, Control, Software

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    Journal: Cell reports

    Article Title: Molecular Competition in G1 Controls When Cells Simultaneously Commit to Terminally Differentiate and Exit the Cell Cycle

    doi: 10.1016/j.celrep.2020.107769

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Rabbit polyclonal anti-PPARG (81B8) , Cell Signaling , Cat# 2443.

    Techniques: Recombinant, Staining, Activity Assay, Plasmid Preparation

    Figure 1. RXRA hot-spot mutations induce the PPAR signaling pathway by activating enhancer/promoters with a canonical PPAR response element. (A) JMSU-1 and 575A cells were transduced with pBABE retrovirus to express indicated RXRA alleles and expression confirmed by western blot (top) or RT- qPCR in triplicate ± SD (data expressed as a fraction of actin signal). (B) Protein coding transcripts up-regulated greater than or equal to twofold Figure 1 continued on next page

    Journal: eLife

    Article Title: Bladder-cancer-associated mutations in RXRA activate peroxisome proliferator-activated receptors to drive urothelial proliferation

    doi: 10.7554/elife.30862

    Figure Lengend Snippet: Figure 1. RXRA hot-spot mutations induce the PPAR signaling pathway by activating enhancer/promoters with a canonical PPAR response element. (A) JMSU-1 and 575A cells were transduced with pBABE retrovirus to express indicated RXRA alleles and expression confirmed by western blot (top) or RT- qPCR in triplicate ± SD (data expressed as a fraction of actin signal). (B) Protein coding transcripts up-regulated greater than or equal to twofold Figure 1 continued on next page

    Article Snippet: A.RXRAwt this paper DKO 431.A organoid line infected with retrovirus carrying pBABE puro RXRA cell line (M. musculus) DKO 431.A.RXRAS427F this paper DKO 431.A organoid line infected with retrovirus carrying pBABE puro RXRA S427F cell line (H. sapiens) JMSU-1 other RRID:CVCL_2081 obtained from Dr. David Solit (MSKCC) cell line (H. sapiens) 575A other RRID:CVCL_7941 obtained from Dr. David Solit (MSKCC) cell line (H. sapiens) UM-UC-3 other RRID:CVCL_1783 obtained from Dr. David Solit (MSKCC) cell line (H. sapiens) Lenti-X 293T Clontech Clontech:632180 cell line (H. sapiens) JMSU-1 RXRA WT this paper JMSU-1 cell line infected with retrovirus carrying pBABE puro RXRA cell line (H. sapiens) JMSU-1 RXRA S427F this paper JMSU-1 cell line infected with retrovirus carrying pBABE puro RXRA S427F cell line (H. sapiens) JMSU-1 RXRA S427Y this paper JMSU-1 cell line infected with retrovirus carrying pBABE puro RXRA S427Y cell line (H. sapiens) 575A RXRA WT this paper 575A cell line infected with retrovirus carrying pBABE puro RXRA cell line (H. sapiens) 575A RXRA S427F this paper 575A cell line infected with retrovirus carrying pBABE puro RXRA S427F antibody anti-PPARG (81B8) (rabbit monoclonal) Cell Signaling Technology Cell Signaling Technology:2443; RRID:AB_823598 (1:1000) antibody anti-PPARD (rabbit monoclonal) Abcam Abcam:ab178866 (1:5000) antibody anti-RXRA (D6H10) (rabbit monoclonal) Cell Signaling Technology Cell Signaling Technology:3085 (1:1200) antibody anti-beta-Actin (mouse monoclonal) Sigma-Aldrich Sigma-Aldrich:A5441; RRID:AB_476744 (1:50000) antibody anti-GAPDH (D16H11) (rabbit monoclonal) Cell Signaling Technology Cell Signaling Technology:5174; RRID:AB_10622025 (1:1000) antibody anti-rabbit IgG, HRP (goat) Cell Signaling Technology Cell Signaling Technology:7074; RRID:AB_2099233 (1:7500) Continued on next page Halstead et al. eLife 2017;6:e30862.

    Techniques: Transduction, Expressing, Western Blot, Quantitative RT-PCR

    Figure 5. RXRA S427F generates PPARD-dependent urothelial growth. (A) Retrovirally transduced organoids from Figure 4 were plated for 7 days in standard media and then treated with the indicated PPARD antagonists (1000 nM ST-247, GSK0660) or PPARG antagonist (100 nM T0070907) for 2 days. Expression of PPAR targets was determined by RT-qPCR in triplicate ±SD and comparison is by Student’s t-test to the RXRAS427F DMSO condition. (See also Figure 5—figure supplement 1)) (B-E) CellTiter-Glo growth assay of indicated organoid lines treated with indicated drugs. Plotted is mean Figure 5 continued on next page

    Journal: eLife

    Article Title: Bladder-cancer-associated mutations in RXRA activate peroxisome proliferator-activated receptors to drive urothelial proliferation

    doi: 10.7554/elife.30862

    Figure Lengend Snippet: Figure 5. RXRA S427F generates PPARD-dependent urothelial growth. (A) Retrovirally transduced organoids from Figure 4 were plated for 7 days in standard media and then treated with the indicated PPARD antagonists (1000 nM ST-247, GSK0660) or PPARG antagonist (100 nM T0070907) for 2 days. Expression of PPAR targets was determined by RT-qPCR in triplicate ±SD and comparison is by Student’s t-test to the RXRAS427F DMSO condition. (See also Figure 5—figure supplement 1)) (B-E) CellTiter-Glo growth assay of indicated organoid lines treated with indicated drugs. Plotted is mean Figure 5 continued on next page

    Article Snippet: A.RXRAwt this paper DKO 431.A organoid line infected with retrovirus carrying pBABE puro RXRA cell line (M. musculus) DKO 431.A.RXRAS427F this paper DKO 431.A organoid line infected with retrovirus carrying pBABE puro RXRA S427F cell line (H. sapiens) JMSU-1 other RRID:CVCL_2081 obtained from Dr. David Solit (MSKCC) cell line (H. sapiens) 575A other RRID:CVCL_7941 obtained from Dr. David Solit (MSKCC) cell line (H. sapiens) UM-UC-3 other RRID:CVCL_1783 obtained from Dr. David Solit (MSKCC) cell line (H. sapiens) Lenti-X 293T Clontech Clontech:632180 cell line (H. sapiens) JMSU-1 RXRA WT this paper JMSU-1 cell line infected with retrovirus carrying pBABE puro RXRA cell line (H. sapiens) JMSU-1 RXRA S427F this paper JMSU-1 cell line infected with retrovirus carrying pBABE puro RXRA S427F cell line (H. sapiens) JMSU-1 RXRA S427Y this paper JMSU-1 cell line infected with retrovirus carrying pBABE puro RXRA S427Y cell line (H. sapiens) 575A RXRA WT this paper 575A cell line infected with retrovirus carrying pBABE puro RXRA cell line (H. sapiens) 575A RXRA S427F this paper 575A cell line infected with retrovirus carrying pBABE puro RXRA S427F antibody anti-PPARG (81B8) (rabbit monoclonal) Cell Signaling Technology Cell Signaling Technology:2443; RRID:AB_823598 (1:1000) antibody anti-PPARD (rabbit monoclonal) Abcam Abcam:ab178866 (1:5000) antibody anti-RXRA (D6H10) (rabbit monoclonal) Cell Signaling Technology Cell Signaling Technology:3085 (1:1200) antibody anti-beta-Actin (mouse monoclonal) Sigma-Aldrich Sigma-Aldrich:A5441; RRID:AB_476744 (1:50000) antibody anti-GAPDH (D16H11) (rabbit monoclonal) Cell Signaling Technology Cell Signaling Technology:5174; RRID:AB_10622025 (1:1000) antibody anti-rabbit IgG, HRP (goat) Cell Signaling Technology Cell Signaling Technology:7074; RRID:AB_2099233 (1:7500) Continued on next page Halstead et al. eLife 2017;6:e30862.

    Techniques: Expressing, Quantitative RT-PCR, Comparison, Growth Assay